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plenti hreluc pgk hygro  (Addgene inc)


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    Addgene inc plenti hreluc pgk hygro
    Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector <t>pLenti</t> <t>hygro</t> FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).
    Plenti Hreluc Pgk Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+hreluc+pgk+hygro/pLenti+HRE-Luc+pGK+Hygro+(Plasmid+%23118706)/pm39823061-164-24-28
    Average 92 stars, based on 5 article reviews
    plenti hreluc pgk hygro - by Bioz Stars, 2026-10
    92/100 stars

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    1) Product Images from "Design and validation of cell-based potency assays for frataxin supplementation treatments."

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.

    Journal: Molecular therapy. Methods & clinical development

    doi: 10.1016/j.omtm.2024.101347

    Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector pLenti hygro FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).
    Figure Legend Snippet: Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector pLenti hygro FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).

    Techniques Used: Expressing, Plasmid Preparation, Western Blot, Molecular Weight, Quantitation Assay, Control

    Related Articles

    Cloning:

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). .. A fragment of 2,782 base pairs (bp) containing the entire FXN gene was excised from pAAV FXN-L using MluI, PmlI, and AatI restriction enzyme digestion and cloned into pLenti HRE-Luc pGK Hygro digested with MluI and PmeI.

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). ..

    Construct:

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). .. A fragment of 2,782 base pairs (bp) containing the entire FXN gene was excised from pAAV FXN-L using MluI, PmlI, and AatI restriction enzyme digestion and cloned into pLenti HRE-Luc pGK Hygro digested with MluI and PmeI.

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). ..

    Expressing:

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). .. A fragment of 2,782 base pairs (bp) containing the entire FXN gene was excised from pAAV FXN-L using MluI, PmlI, and AatI restriction enzyme digestion and cloned into pLenti HRE-Luc pGK Hygro digested with MluI and PmeI.

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). ..

    Control:

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). .. A fragment of 2,782 base pairs (bp) containing the entire FXN gene was excised from pAAV FXN-L using MluI, PmlI, and AatI restriction enzyme digestion and cloned into pLenti HRE-Luc pGK Hygro digested with MluI and PmeI.

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). ..

    Clone Assay:

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). .. A fragment of 2,782 base pairs (bp) containing the entire FXN gene was excised from pAAV FXN-L using MluI, PmlI, and AatI restriction enzyme digestion and cloned into pLenti HRE-Luc pGK Hygro digested with MluI and PmeI.

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). ..

    Plasmid Preparation:

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). .. A fragment of 2,782 base pairs (bp) containing the entire FXN gene was excised from pAAV FXN-L using MluI, PmlI, and AatI restriction enzyme digestion and cloned into pLenti HRE-Luc pGK Hygro digested with MluI and PmeI.

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.
    Article Snippet: MEFs were seeded at 5 103–5 104 cells per well in 96-well plates and cultured for 48 h. ATP levels were measured using the Luminescent ATP Detection Assay Kit (Abcam, #ab113849) following the manufacturer’s recommendations. .. Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706). ..



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    plenti hreluc pgk hygro  (Addgene inc)
    92
    Addgene inc plenti hreluc pgk hygro
    Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector <t>pLenti</t> <t>hygro</t> FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).
    Plenti Hreluc Pgk Hygro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+hreluc+pgk+hygro/pLenti+HRE-Luc+pGK+Hygro+(Plasmid+%23118706)/pm39823061-164-24-28
    Average 92 stars, based on 1 article reviews
    plenti hreluc pgk hygro - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector pLenti hygro FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).

    Journal: Molecular therapy. Methods & clinical development

    Article Title: Design and validation of cell-based potency assays for frataxin supplementation treatments.

    doi: 10.1016/j.omtm.2024.101347

    Figure Lengend Snippet: Figure 3. Stable expression of exogenous human FXN in MUT MEFs (A) A schematic of the lentivirus vector pLenti hygro FXN encoding a human miniFXN gene. The miniFXN gene used in experiments contains fragments of the endogenous FXN 50UTR, promoter and intron 1, and all five exons of the gene. (B) Representative western blot demonstrating expression of exogenous FXN. The miniFXN encodes a C-terminal HA tag that results in a slightly greater molecular weight, as detected by western blot. (C) Quantitation of endogenous Fxn (*) and exogenous FXN by western blot. Gapdh was used as a normalization control. MUT+FXN designates cells expressing exogenous, human FXN. The experiment was performed three independent times. Results are plotted as mean values with standard deviations; (**p < 0.001, ***p < 0.0001).

    Article Snippet: Cloning and packaging of pLenti hygro FXN-L A miniFXN construct expressing human FXN under the control of endogenous regulatory elements was cloned into the pLenti HRELuc pGK Hygro (Addgene plasmid #118706).

    Techniques: Expressing, Plasmid Preparation, Western Blot, Molecular Weight, Quantitation Assay, Control